Yes. NV2 has been tested with Windows 10 and they are no problems with operating NV2 on this operating system. There are no tweaks or caveats.
NV2 runs on Windows 2000 and above. In a multi-user environment, some machines can be a combination of various Windows versions. The NV2 server is not limited to the Windows version connect from. You can upgrade your machines in any manner that you see fit.
I hope this helps,
Regards to All,